سمپوزیوم منطقه ای نوآوری در علم و فناوری

صفحه اصلی / دومین سمپوزیوم منطقه ای نوآوری در علم و فناوری

Targeted Identification of biopolymer sphingan producers: RAPD-PCR analysis and the development of degenerate PCR primers

نویسندگان :

Monir-sadat Shakeri¹

1- Research Institute of Food science And Technology

کلمات کلیدی :

Sphingomonas identification،Cluster analysis،RAPD-PCR،MCA

چکیده :

Sphingomonas strains are the main focus for producing various microbial polysaccharides such as welan, gellan, and diutan, all of which are based on sphingan. These substances and their different forms are commonly used in various fields, including medicine, food, and the petroleum industry. It is crucial to identify local strains in order to obtain high-yield strains. In this study, screening of indigenous Sphingomonas isolates obtained from environmental sources including soil and water was conducted using degenerate primers designed from the pgmG gene, along with RAPD-PCR technique. The strains were precisely identified by sequence analysis of the 16SrDNA region. The study found 44 bacterial isolates classified as gram-negative bacilli that showed positive results for the catalase test. The isolates were analyzed for RAPD patterns, which led to the identification of 14 subclusters with a similarity of 45%. The identification of ten distinct Sphingomonas strains was achieved through sequencing the 16SrDNA within these subclusters. The designed primers were assessed to determine the target band, revealing that all ten strains produced the expected band and have the ability to produce sphingan-based biopolymers. Combining PCR with degenerate primers and RAPD analysis provides a systematic and accurate method for carrying out epidemiological research on sphingomonas strains

لیست مقالات

لیست مقالات بایگانی شده

Application of Lasers in Precision Volume Measurement of Liquids

Ayda Sarkaki - Mohammad Reza Rashidian Vaziri - Seyed Mohammad Ali Abadi - Hadi Rastegar Mogahddam Rezaion - Mina Mayeli

Design of DNA Origami-Heparin Nanostructures for the Development of Viral Therapies

Sadegh Dastorani - Mahmoud Shariati - Reza Hasanzadeh Ghasemi

طراحی نیم و تمام جمع‌کننده کواترنری مبتنی بر ترانزیستورهای نانو لوله کربنی

سبحان آقامعلیزاده - جواد جاویدان

Human health risk assessment of atmospheric dust in Dargaz area NE-Iran

Faeze Khodaverdizade - Mohammad Hosein Mahmudy Gharaie - Roozbeh Shad - Hossein Sadeghi

Types of lasers and their applications in burn patients

Mahsa Goharara

A Whole-cell amperometric biosensor based on green microalgal enzyme for water pollution monitoring

Sima Iravani - Hossein Ahmadzadeh - Halimeh-sadat Sajjadizadeh - Gholamhossein Rounaghi

Optimized Statistical Analysis of Factors Influencing the Growth of Carbon Nanotubes in Chemical Vapor Deposition (CVD) Processes

Elahe Khosravifard - Mohammad Taghi Hamed Mosavian - Morteza Maghrebi

Biodegradation of Deltamethrine in soil by co-culture of Pseudomonas aeruginosa and Rhodococcus pyridinivorans

Neda Sharafi - َAli Makhdoumi - Mohamad Hosein Mahmudy Gharaie - Hosein Ahmadzadeh - Zahra Khamar

Bioremediation of Pb(II) and Ni(II) by live Dunaliella salina microalgae: A green dynamic approach

Somayyeh Ziaei - Hossein Ahmadzadeh - Zarrin Es’haghi

Integration of High-Definition simultaneous EEG and fNIRS recording system

Mojtaba Pourdara - Mostafa Khavanin Zadeh - Javad Safaie

بیشتر