Regional Symposium on Innovation in science and technology

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Rapid detection of Enterococcus faecalis using extrinsic fluorescence

Authors :

Abolfazl Zanghaei¹ Ali Ameri² Vahid Soheili³ Ali Hashemi⁴ Saeed Karima⁵ Hossein Ghanbarian⁶ Kiarash Ghazvini⁷

1- دانشگاه علوم پزشکی مشهد 2- دانشگاه علوم پزشکی شهید بهشتی 3- دانشگاه علوم پزشکی مشهد 4- دانشگاه علوم پزشکی شهید بهشتی 5- دانشگاه علوم پزشکی شهید بهشتی 6- دانشگاه علوم پزشکی شهید بهشتی 7- دانشگاه علوم پزشکی مشهد

Keywords :

Enterococcus faecalis،Fluorescence،Rapid detection،spectroscopy

Abstract :

Enterococcus faecalis (E. faecalis) is a gram-positive bacterium associated with humans as a member of gut microflora. The approaches based on the intracellular and extracellular metabolites are of major interest. Four strains of bacteria, including standard and clinical samples of E. faecalis, Staphylococcus aureus, Escherichia coli, and Shigella flexneri, with seven levels of concentrations, were included in this study. After 30 minutes of incubation, the bacteria were treated with 2′-7′-dichlorodihydrofluorescein diacetate (H2-DCF-DA), and subsequently, after 30 minutes, the samples were scanned by a fluorescence spectrometer. The intensity greater than a threshold, indicates the detection of both clinical and standard samples of E. faecalis with a concentration of 105 CFU/mL or higher. Regarding the specific enzymes of E. faecalis and its high level of redox reactions, a fast, a low-cost method, using H2-DCF-DA, is presented. The fluorescence emission after 60 minutes and greater than a threshold indicates the detection of E. faecalis.

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