Regional Symposium on Innovation in science and technology

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A Whole-cell amperometric biosensor based on green microalgal enzyme for water pollution monitoring

Authors :

Sima Iravani¹ Hossein Ahmadzadeh² Halimeh-sadat Sajjadizadeh³ Gholamhossein Rounaghi⁴

1- دانشگاه فردوسی مشهد 2- دانشگاه فردوسی مشهد 3- دانشگاه فردوسی مشهد 4- دانشگاه فردوسی مشهد

Keywords :

whole-cell biosensor،microalgae،water monitoring

Abstract :

Abstract— Engineering of an efficient, sensitive, and easy-to-operate biosensor emerges as a rapid devices and green method to address the water pollution monitoring. In the present work, a whole-cell amperometric biosensor based on native microalgae of Iran was constructed to investigate the biotoxicity of heavy metal ions in freshwater. The biosensor responses were based on chronoamperometric currents generated by enzyme alkaline phosphatase (ALP) activity on the cell membrane. Heavy metal ions have an inhibition effect on ALP therefore; a decrease in the general current can be utilized to determine the toxicity degree of these ions. The biosensor showed a high sensitivity with LOD of 0.1 ppb for heavy metal ions.